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With lattice structured illumination microscopy, live, 3D multicolor acquisitions at resolutions below 100 nm have become possible. By using standard dyes and fluorescent proteins, this technology provides its users with an easy imaging approach with the speed and light dose of widefield imaging - but with superresolution results. One of the first users of the ZEISS implementation of this technology (SIM²) is Peter O'Toole from the University of York in UK. In this video, he and his colleagues s
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